Triterpene (asiaticoside) dosage

The analysis of triterpenes , and more specifically asiaticosides, is an essential step for manufacturers of nutraceuticals, food, animal nutrition, and cosmetics. These natural molecules, characteristic of the medicinal plant Centella asiatica , are recognized for their biological properties, notably their role in cell regeneration and wound healing. Their presence and concentration must be precisely controlled to guarantee the quality, safety, and conformity of finished products.

Understanding asiaticosides and their importance

Asiaticosides belong to the family of triterpene saponins. They are primarily extracted from Centella asiatica , a plant used for centuries in traditional Asian medicine. These compounds are particularly studied for:

• Their healing and restorative properties
• Their effect on microcirculation and vascular health
• Their role in modulating collagen production
• Their interest in anti-aging cosmetics and dermatology

These characteristics explain the widespread use of asiaticosides in food supplements, pharmaceutical formulations and cosmetic care.

Analytical methods for the determination of asiaticosides

The quantification of asiaticosides relies on liquid chromatography techniques adapted to complex matrices. The two main methods are:

• HPLC (high-performance liquid chromatography) , which allows for precise separation and quantification of triterpenes in plant extracts, powders or cosmetic formulations.
• LC-MS/MS (liquid chromatography coupled with tandem mass spectrometry) , offering increased sensitivity and specific identification, ideal for difficult matrices or low concentrations.

These techniques guarantee a reliable and reproducible analysis, essential to meet regulatory requirements and industry quality standards.

Sectors concerned by triterpene analysis

The measurement of asiaticosides is of interest to various industrial sectors:

• Nutraceuticals : development of food supplements based on Centella asiatica or standardized extracts.
• Human nutrition : integration of triterpenes into functional products for their potential health benefits.
• Animal nutrition : formulation of products enriched with plant extracts to improve animal health and productivity.
• Cosmetics and dermatology : evaluation of repairing and anti-aging active ingredients in creams, serums and lotions.
• Phytotherapy and pharmacology : validation of plant extracts used in therapeutic products.

Typically analyzed matrices

Asiaticoside analysis is performed in a wide variety of matrices, reflecting the versatility of these compounds:

• Plant extracts of Centella asiatica
• Powders and capsules intended for nutraceutical use
• Dermatological creams and treatments
• Liquid or solid food supplements
• Functional foods and enriched formulations

Regulatory and quality issues

Control of asiaticosides is essential for:

• Verify regulatory compliance, particularly in the European Union and in international markets
• Ensuring the reproducibility and standardization of plant extracts used
• Ensuring the transparency and reliability of health or cosmetic claims
• Reduce the risks of contamination, fraud or quality variation between batches

In a growing market where traceability requirements are increasingly stringent, triterpene analysis is an essential tool for securing production chains and strengthening consumer confidence.

YesWeLab support for your asiaticoside analyses

YesWeLab provides you with access to its network of partner laboratories specializing in chromatography and spectrometry. Our partners are selected for their expertise and accreditations (ISO 17025, COFRAC), guaranteeing reliable and recognized results.

Thanks to our digital platform, you can:

• Submit your samples in just a few clicks
• Track the progress of your analyses
• Receive your results quickly and securely

Our scientific team will guide you in choosing the method best suited to your needs and matrices. Since 2020, numerous manufacturers, distributors, and engineering firms have entrusted us with their analyses to ensure the success of their projects and compliance with regulatory standards.

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