Assay of processed animal proteins (PAT)

The analysis of processed animal proteins (PAP) identifies the presence of animal-derived proteins in compound feeds, meals, raw materials, or finished products . This analysis is essential to guarantee product safety risk of bovine spongiform encephalopathy (BSE) , and ensure regulatory compliance in the animal nutrition and "animal protein-free" food .

Processed animal proteins (PAPs) are derived from the processing of animal tissues (meat, fish, bones, blood, etc.) and their use is strictly regulated by European legislation (Regulation (EU) No. 999/2001 and No. 56/2013). Analysis is therefore essential to guarantee traceability and the strict separation of plant and animal supply chains .

Analytical methods: PCR, microscopy and ELISA

The choice of method depends on the nature of the product and the level of detection required:

• PCR (Polymerase Chain Reaction) : reference technique allowing the specific detection of animal DNA (ruminants, pigs, poultry, fish, etc.) even in trace amounts.
• Optical microscopy : traditional standardized method (EC regulation 51/2013) allowing the morphological identification of fragments of bone, feathers or scales in compound foods.
• ELISA (immuno-enzymatic) : used for the quantification of specific proteins of animal origin by antigen-antibody reaction.

These methods comply with European guidelines and ISO 17025 standards , ensuring the reliability of results for official controls and export.

Applications and regulatory challenges

The measurement of PAT (parathyroid hormone) is applicable in many contexts:

• Animal nutrition : detection of cross-contamination in flours, oilseed cakes and compound feeds.
• Food industry vegetarian ” or “ vegan products .
• Quality control and compliance : adherence to regulatory requirements and certifications free of animal matter.
• Health security : prevention of risks related to unconventional transmissible pathogens (BSE).

This analysis can be combined with animal DNA quantification by quantitative PCR or GMO detection to enhance the traceability of raw materials.

Affected matrices

YesWeLab's partner laboratories perform PAT assays on:

• Compound feeds and premixes for animals
• Agricultural raw materials (flours, oilseed cakes, oils)
• Finished products intended for animal or human consumption
• Formulations certified free of animal products

Each protocol is adapted according to the matrix , the applicable regulations and the required level of sensitivity

YesWeLab expertise

At YesWeLab , we work with a network of ISO 17025 and COFRAC accredited laboratories , specializing in molecular biology and food testing .

Our scientific team assists you in choosing detection methods , preparing samples and fully monitoring analyses via our secure digital platform .

Since 2020, YesWeLab has been supporting food manufacturers, distributors, agricultural cooperatives and research offices in their regulatory and health controls related to processed animal proteins.

Order a PAT analysis

Do you want to verify the presence of processed animal proteins in your products or guarantee 100% plant-based manufacturing?
Submit your request via our platform or contact our scientific team for personalized support .

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