Determination of aflatoxins B1, B2, G1 and G2

The measurement of aflatoxins B1, B2, G1, and G2 allows for the detection and quantification of these highly toxic mycotoxins produced by fungi of the genus Aspergillus (primarily A. flavus and A. parasiticus ). These compounds are known for their carcinogenic, mutagenic, and hepatotoxic properties , and are subject to strict regulations in products intended for human and animal consumption.

The analysis is carried out by high-performance liquid chromatography (HPLC), liquid chromatography coupled with mass spectrometry (LC-MS/MS) or by enzyme-linked immunosorbent assay (ELISA), depending on the level of precision and regulatory thresholds required.

Principle and operation of the method

The sample (cereals, dried fruits, vegetable powders, animal feed, etc.) is first subjected to extraction using specific solvents to isolate aflatoxins.

• The ELISA method is based on antigen-antibody recognition, suitable for routine analyses and rapid screening.
• HPLC with fluorescence detection allows for precise quantification of the four main aflatoxins.
• LC -MS/MS offers optimal sensitivity and specificity, enabling the detection of mycotoxins in trace amounts, even in complex matrices.

The results are expressed in micrograms per kilogram (µg/kg), in accordance with the maximum limits set by Regulation (EC) No 1881/2006 and its updates, which govern the maximum levels of aflatoxins in food and feed.

Analytical performance and related analyses

HPLC, LC-MS/MS and ELISA methods offer:

• high sensitivity (detection limits on the order of ppb),
• high specificity for differentiating aflatoxins B1, B2, G1 and G2,
• and inter-laboratory reproducibility ensuring the reliability of the results.

For a complete assessment of mycotoxic risk, aflatoxin testing can be supplemented by:

• the measurement of ochratoxin A ,
• the dosage of fumonisins,
• the dosage of zearalenones,
• or the measurement of trichothecenes (DON, T-2, HT-2)) .

These combined analyses allow for comprehensive monitoring of fungal contamination in agricultural, food and nutraceutical products.

Applications and areas of use

Aflatoxin testing is essential in several sectors:

• Human food : quality control of cereals, flours, dried fruits, spices and processed products to ensure regulatory compliance and consumer safety.
• Animal nutrition : monitoring of raw materials and compound feeds to prevent chronic toxicity and effects on animal health.
• Nutraceuticals and plants : verification of the purity of plant extracts and botanical powders used in food supplements.
• Export and regulatory compliance : control of batches subject to strict international regulations, particularly in the European Union.

These analyses guarantee the sanitary and regulatory compliance of products throughout the production and distribution chain.

The advantages of the YesWeLab service

YesWeLab relies on a network of partner laboratories specializing in the detection of contaminants and mycotoxins, certified ISO 17025 and accredited by COFRAC. Each project is entrusted to a laboratory selected according to the matrix, the applicable regulations, and the most suitable analytical method.

Thanks to its digital analysis platform, YesWeLab simplifies the management of your regulatory analyses: request quotes, track samples, and receive results online. Since 2020, numerous manufacturers, producers, and formulators have trusted us for their food and nutraceutical safety testing.

For any specific request regarding the measurement of aflatoxins B1, B2, G1 and G2, contact our scientific team to obtain a personalized quote and support tailored to your analytical objectives.