Dosage of aescins (as. hederacoside c)

Aescins are a mixture of triterpene saponins extracted mainly from the seeds and bark of the horse chestnut tree ( Aesculus hippocastanum ). Among them, hederacoside C is one of the most representative and studied compounds. These bioactive molecules are recognized for their venotonic and anti-inflammatory properties, explaining their traditional use in the treatment of chronic venous insufficiency and circulatory disorders. The quantification of aescins in plant extracts and finished products is essential to ensure the standardization, traceability and therapeutic efficacy of formulations.

Analytical methods for the determination of aescins

The determination of aescins is based on high-precision chromatographic techniques.

• HPLC-DAD (High Performance Liquid Chromatography with Diode Array Detection) : a method commonly used to separate and quantify the different aescin fractions in horse chestnut extracts.
• LC-MS (Liquid Chromatography-Mass Spectrometry) : provides enhanced sensitivity and specific identification of aescin derivatives, including hederacoside C.

These methods provide reliable results that comply with regulatory requirements. They are part of the range of analyses offered by YesWeLab, alongside HPLC and LC-MS/MS, which are used for many plant bioactive substances.

Applications and sectors concerned

The dosage of aescins is particularly relevant in the following sectors:

• Nutraceuticals : for the standardization of circulatory food supplements.
• Cosmetics : for formulations intended to improve skin microcirculation and reduce signs of fatigue.
• Pharmaceutical research and R&D : for the evaluation of the biological and pharmacological effects of horse chestnut extracts.

This service is part of a broader approach to promoting plant-based active ingredients, in the same way as the dosage of gymnemic acids , trigonelline or theanine , also offered by YesWeLab for the nutraceutical sector.

Matrices analyzed for aescins

Partner laboratories perform aescin assays on different types of matrices: standardized horse chestnut extracts, plant powders, food supplements in the form of capsules, tablets or liquid solutions, and cosmetic products for circulatory purposes. The analytical method is chosen according to the matrix, in order to guarantee optimal extraction and reproducible results.

Challenges of standardization and traceability

The aescin content varies greatly depending on the origin of the raw material, the extraction processes and the manufacturing conditions. Laboratory determination allows: to verify the actual concentration of claimed active ingredients, to compare the quality and consistency between different batches, to ensure complete traceability of the product, to guarantee compliance with the standards in force for marketing. These challenges are similar to those encountered for other bioactive molecules such as tryptamine or tyramine , which also require precise quantification to guarantee the safety and consistency of the products.

YesWeLab's expertise in aescine analysis

YesWeLab collaborates with a network of more than 200 partner laboratories in France and Europe, selected for their scientific expertise and accreditations (ISO 17025, COFRAC, etc.). Each request is analyzed to determine the most appropriate method based on the samples and regulatory constraints. Thanks to our digital platform, you benefit from a simplified and transparent service: online quote request, sample tracking, and receipt of results within the agreed deadlines. Since 2020, numerous manufacturers, distributors, and design offices have entrusted us with their analyses to ensure the reliability of their products and meet market expectations.

Take action

Do you want to guarantee the quality and compliance of your horse chestnut extracts or circulatory supplements? Entrust your samples to YesWeLab for reliable and accurate analysis. Request a quote online now for your aescin (hederacoside C) assay.