Dosage of aescins (as. hederacoside c)

Aescins are a mixture of triterpene saponins extracted primarily from the seeds and bark of the horse chestnut tree ( Aesculus hippocastanum ). Among them, hederacoside C is one of the most representative and studied compounds. These bioactive molecules are recognized for their venotonic and anti-inflammatory properties, explaining their traditional use in the treatment of chronic venous insufficiency and circulatory disorders. Quantifying aescins in plant extracts and finished products is essential to ensure the standardization, traceability, and therapeutic efficacy of formulations.

Analytical methods for the determination of aescins

The quantification of aescins relies on high-precision chromatographic techniques.

• HPLC-DAD (High Performance Liquid Chromatography with Diode Array Detection) : a method commonly used to separate and quantify the different fractions of aescins in horse chestnut extracts.
• LC-MS (Liquid Chromatography coupled with Mass Spectrometry) : offers enhanced sensitivity and specific identification of aescin derivatives, notably hederacoside C.

These methods provide reliable results that comply with regulatory requirements. They are part of the range of analyses offered by YesWeLab, alongside HPLC and LC-MS/MS, which are used for many plant bioactive substances.

Applications and sectors concerned

Aescin assays are particularly relevant in the following sectors:

• Nutraceuticals : for the standardization of circulatory food supplements.
• Cosmetics : for formulations intended to improve skin microcirculation and reduce signs of fatigue.
• Pharmaceutical research and R&D : for the evaluation of the biological and pharmacological effects of horse chestnut extracts.

This service is part of a broader approach to enhancing the value of plant-based ingredients, similar to the measurement of gymnemic acids , trigonelline or theanine , also offered by YesWeLab for the nutraceutical industry.

Matrices analyzed for aescines

Our partner laboratories perform aescin assays on various types of matrices: standardized horse chestnut extracts, plant powders, dietary supplements in capsule, tablet, or liquid solution form, and cosmetic products intended to support circulation. The analytical method is selected based on the matrix to ensure optimal extraction and reproducible results.

Challenges of standardization and traceability

The aescin content varies considerably depending on the origin of the raw material, the extraction processes, and the manufacturing conditions. Laboratory testing allows for: verification of the actual concentration of the claimed active ingredients, comparison of quality and consistency between different batches, ensuring complete product traceability, and guaranteeing compliance with applicable market standards. These challenges are similar to those encountered with other bioactive molecules such as tryptamine or tyramine , which also require precise quantification to guarantee product safety and consistency.

YesWeLab's expertise in aesthetic analysis

YesWeLab collaborates with a network of over 200 partner laboratories in France and Europe, selected for their scientific expertise and accreditations (ISO 17025, COFRAC, etc.). Each request is analyzed to determine the most suitable method based on the samples and regulatory requirements. Thanks to our digital platform, you benefit from a simplified and transparent service: online quote requests, sample tracking, and timely delivery of results. Since 2020, numerous manufacturers, distributors, and engineering firms have entrusted us with their analyses to ensure the reliability of their products and meet market expectations.

Take action

Do you want to guarantee the quality and compliance of your horse chestnut extracts or circulatory supplements? Entrust your samples to YesWeLab for reliable and precise analysis. Request a quote online now for your aescin (hederacoside C) assay.