DNA assay (detection and identification of terrestrial vertebrates (up to 21 of the most commonly sought species) and fish

DNA testing for the detection and identification of terrestrial vertebrates and fish is a reference method for verifying the traceability of animal raw materials in food products, animal feed, and nutritional supplements.
This analysis can identify up to 21 commonly sought vertebrate species, including beef, pork, poultry, sheep, horse, fish, and other species used or prohibited according to regulations.

Based on PCR (Polymerase Chain Reaction) and DNA sequencing (NGS – Next Generation Sequencing), this method makes it possible to detect the presence, even at very low levels, of animal DNA in complex matrices.

Why perform a DNA test?

DNA analysis is essential in several industrial and regulatory contexts:

• To guarantee the traceability and authenticity of food products and animal feed.
• Prevent fraud and species substitution, particularly in meat products, animal meal, protein supplements and processed products.
• Comply with European regulations prohibiting certain raw materials of animal origin depending on the sector (in particular Directive 2001/18/EC and Regulation (EC) No 1069/2009).
• Ensure ethical or religious compliance (halal, kosher, vegetarian) by identifying the undeclared presence of certain species.
• Assess cross-contamination in production and processing chains.

This analysis therefore addresses issues of security, transparency and quality, while strengthening consumer confidence.

Analytical methods used

At YesWeLab , DNA testing relies on cutting-edge technologies combining targeted gene amplification and next-generation sequencing .

Multiplex PCR (Polymerase Chain Reaction)
This technique allows the simultaneous amplification of several genetic sequences specific to different species. Using targeted primers, PCR identifies the presence of animal DNA, even in trace amounts (less than 0.1%).
It is used for rapid authenticity and compliance checks, particularly in food matrices and animal feed.

Next Generation Sequencing (NGS)
High-throughput sequencing enables comprehensive and precise identification of the species present in a sample. Unlike targeted PCR, NGS analyzes thousands of genetic fragments simultaneously, making it particularly well-suited for processed or complex products .
This approach offers unparalleled sensitivity and reliability for the complete characterization of a product's DNA profile.

The analyses are carried out according to protocols conforming to the ISO 17025 , ISO 21571 and ISO 24276 , guaranteeing the traceability, reproducibility and reliability of the results .

Industrial and sectoral applications

Animal nutrition and feed:
Control of animal meal, premixes, and compound feeds to verify the absence of unauthorized species. An essential tool for manufacturers concerned with compliance with European and national regulations.

Agribusiness and meat processing:
Verification of the composition of meat products, deli meats, prepared meals, and industrial preparations. DNA analysis makes it possible to identify fraudulent substitutions (e.g., the presence of horse meat in a product labeled “pure beef”).

Food supplements and protein products:
Control of formulations based on animal proteins (collagen, gelatin, hydrolysates) to ensure consistency with composition or origin claims.

Environment and traceability:
Identification of species in environmental matrices, waters or biological substrates for ecological monitoring and characterization of biological origin.

Affected matrices

• Animal feed, flours and premixes
• Meat products, prepared meals, deli meats
• Protein and nutraceutical supplements
• Environmental or biological samples

Recommended Related Analyses

For comprehensive control of product origin and composition, YesWeLab recommends combining DNA testing with:
Animal protein authenticity analysis to complement raw material verification;
total protein analysis to correlate DNA profile with organic matter content;
and GMO (transgenic DNA) detection to assess regulatory compliance for plant and animal-derived products.

Why entrust your analysis to YesWeLab?

YesWeLab relies on a network of ISO 17025 accredited laboratories, experts in molecular biology and DNA authentication. Our partners have the latest equipment for quantitative PCR, multiplex PCR, and next-generation sequencing (NGS).
Thanks to our digital platform, you can submit your samples, track your analyses, and retrieve your results quickly, securely, and centrally.
Our experts also support manufacturers in implementing DNA control plans tailored to their industry and compliance needs.