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Digitoxigenin (cardiac glycoside) dosage

Digitoxigenin (cardiac glycoside) dosage

Technique(s)
Categories: ,
Sector of activity
Expertise

Physicochemical, Nutritional

CAS No.

143-62-4

Description

Digitoxigenin is a steroidal aglycone derived from the degradation of cardenolide glycosides such as digitoxin, naturally present in plants such as foxglove (Digitalis spp.). Although its pharmaceutical use is regulated, its dosage is relevant in non-medical contexts to control the presence of residues in plant extracts, assess the purity of technical substances or in environmental toxicology studies.

Digitoxigenin assay is an analysis performed by liquid chromatography (HPLC or UPLC), generally coupled with mass spectrometry (LC-MS/MS) to ensure specific and sensitive detection, even at trace levels. This assay is suitable for plant matrices, natural extracts or technical supports requiring precise identification of active steroidal compounds.

 

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