Dosage of dicoumarol (dicoumarin)

Dicoumarol (or dicoumarin , CAS No. 66-76-2) is a natural anticoagulant produced by the oxidative fermentation of coumarin . It was initially identified in sweet clover ( Melilotus officinalis ) , a forage plant whose improper storage can lead to the formation of this biologically active compound. Historically used as a precursor to warfarin-type anticoagulants , dicoumarol is now primarily sought after for quality control and safety of plant-based raw materials and nutraceutical products.

Dicoumarol testing allows for the evaluation of the concentration of this compound with pharmacological effects in extracts, powders or finished products, in order to ensure the safety of use and regulatory compliance of herbal products.

Analytical methods: HPLC and LC-MS

Dicoumarol analysis is performed using high-precision chromatographic methods :

• HPLC (high-performance liquid chromatography) : a reliable technique for the separation and quantification of dicoumarol in complex plant-based extracts.
• LC-MS (liquid chromatography coupled with mass spectrometry) : allows specific and sensitive detection of dicoumarol, even at very low concentrations, and precise structural identification .

These methods guarantee reproducible quantification and comply with the requirements of good analytical practices (GLP) nutraceutical and phytopharmaceutical contexts .

Industrial and scientific applications

Dicoumarol dosage is applicable to many areas:

• Nutraceuticals and phytotherapy : quality control of extracts of Melilotus officinalis and other plants rich in coumarins.
• Food and feed safety : detection of dicoumarol in raw materials to avoid any risk of animal poisoning.
• Formulation of food supplements : verification of conformity and absence of adverse effects.
• Pharmacological research : studies on the interactions between coumarin compounds and anticoagulant activity.

For a complete characterization, this analysis can be combined with the measurement of coumarin or warfarin (synthetic coumarin) , in order to evaluate the cumulative profile of compounds with anticoagulant activity.

Affected matrices

Our partner laboratories perform dicoumarol assays on several types of matrices:

• Plant extracts of Melilotus officinalis or other Fabaceae
• Powders and natural raw materials
• Food supplements and nutraceutical formulations
• Raw materials intended for animal nutrition

Each protocol is adapted to the matrix and the required level of sensitivity , guaranteeing reliable results that comply with current regulations (EU, EFSA, ISO).

YesWeLab expertise

At YesWeLab , we collaborate with a network of over 200 partner laboratories , located in France, Europe and internationally , rigorously selected for their analytical expertise and their ISO 17025 and COFRAC accreditations .

Our scientific team assists you in choosing the appropriate analytical method , selecting the specialized laboratory , and monitoring your analyses via our digital platform . This platform allows you to centralize your requests , obtain quick quotes , and track your samples in real time .

Since 2020, many manufacturers, formulators and distributors have trusted us to ensure the safety and compliance of their plant extracts and nutraceutical products.

Order a dicoumarol (dicoumarin) analysis

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