Daidzin dosage

Daidzin is a glycosylated isoflavone belonging to the phytoestrogen family, naturally present in soy, red clover, and other legumes. It is the glycosidic form of daidzein, a bioactive molecule well known for its antioxidant, anti-inflammatory, and estrogen-like effects. Widely used in nutraceuticals, daidzin plays a central role in the formulation of dietary supplements for menopause, bone health, and metabolic well-being.

Daidzin measurement is crucial for ensuring the standardization of plant extracts, controlling the quality of raw materials, and monitoring enzymatic transformations in enriched formulations. It also allows for the verification of the presence of daidzin in active ingredients derived from fermentation, extraction, or concentration processes.

Why analyze daidzin?

The analysis of daidzin is part of an approach of:

• Standardization of soy or red clover extracts used as food supplements
• Control of the composition of functional and phytochemical products
• Validation of health claims (particularly on effects linked to menopause or osteoporosis)
• Evaluation of the antioxidant and hormonal efficacy of a natural ingredient
• Monitoring the enzymatic conversion of daidzin to daidzein or equol, particularly in fermented products

This analysis is also relevant in the context of pharmacokinetic research, galenic development, or control of supplier batches.

Analytical techniques used

The dosage of daidzin is generally carried out by high-performance chromatographic techniques, adapted to the complexity of plant matrices or finished products:

• HPLC-DAD : High-performance liquid chromatography with UV detection, ideal for the quantification of daidzin in concentrated extracts.
• LC-MS/MS : Liquid chromatography coupled with mass spectrometry, recommended for complex matrices, multi-component products or very low concentration assays.

An extraction step (hydroalcoholic or enzymatic) may be necessary to release daidzin in certain matrices, followed by purification by SPE or filtration.

Analyzed matrices

• Soy extracts, red clover, kudzu
• Food supplements (capsules, powders, tablets)
• Plant ingredients enriched with isoflavones
• Fermented soy products (tempeh, miso)
• Cosmetic ingredients based on phytoestrogens

Industrial applications

The dosage of daidzin is integrated into the processes of:

• Quality control and regulatory compliance
• Development of new functional ingredients
• Labeling validation (guaranteed isoflavone levels)
• Stability and bioavailability studies
• Monitoring of enrichment or fermentation processes

Why choose YesWeLab for daidzin analysis?

YesWeLab gives you access to a network of partner laboratories specialized in phytochemistry, accredited ISO 17025 or COFRAC, capable of handling the analysis of daidzin in complex or regulated matrices.

With YesWeLab, you benefit from:

• Access to HPLC or LC-MS/MS reference techniques
• Deadlines adapted to your project constraints
• Tailor-made support from our scientific team
• Technical support from the moment your request is submitted

Our digital platform simplifies the management of your samples, from ordering to receiving results, with personalized monitoring throughout the process.

Recommended internal links

To complete your analyses of isoflavones and associated compounds, we also recommend:

• Dosage of daidzein : the aglycone form derived from daidzin
• Genistein dosage : another major isoflavone in soy
• Total isoflavone assay : for global quantification of phytoestrogens

Since 2020, YesWeLab has been supporting manufacturers, formulators, and distributors in the quality control of their plant-based ingredients. Contact our team to learn more or receive a proposal tailored to your analytical needs.