Daidzein ( CAS: 486-66-8) is an aglycone isoflavone naturally present in soybeans , red clover , kudzu , and other legumes. It is formed by enzymatic hydrolysis of its glycosylated form, daidzin , particularly during digestion or fermentation. Widely studied for its , anti-inflammatory , and phytoestrogenic effects , daidzein is a key marker in evaluating the efficacy of soy-based ingredients.
Its dosage is essential for the standardization of plant extracts , the quality control of food supplements , or even the evaluation of bioavailability in the context of clinical or nutritional research.
Why dose daidzein?
The dosage of daidzein is part of various industrial or scientific approaches:
- Standardizing plant extracts rich in isoflavones
- Monitoring the enzymatic conversion of daidzin to daidzein in fermented products (miso, tempeh, natto)
- Verify the conformity of functional claims on products enriched with phytoestrogens
- Evaluate the bioavailability and metabolism of isoflavones in pharmacokinetic studies
- Compare the isoflavone content of different raw materials or suppliers
This analysis is recommended for formulators, nutraceutical brands, research laboratories and manufacturers of plant-based active ingredients.
Proposed analytical methods
The analysis of daidzein is carried out by liquid chromatography , using two main techniques:
- HPLC-DAD: allows the quantification of daidzein by UV detection, particularly suitable for concentrated extracts and simple matrices.
- LC-MS/MS: liquid chromatography coupled with mass spectrometry for increased sensitivity, even at very low concentrations or in complex matrices.
A preliminary extraction (hydroalcoholic or enzymatic) is often necessary, followed by purification or SPE treatment, depending on the nature of the sample.
Affected matrices
Daidzein dosage can be performed on many matrices:
- Soybean extracts, kudzu, red clover
- Food supplements (capsules, powders, tablets)
- Fermented products (tofu, tempeh, miso, natto)
- Liquid or concentrated formulations intended for supplementation
- Functional ingredients or botanical raw materials
Industrial applications
- Formulation and validation of isoflavone-based supplements
- Supplier batch tracking and traceability of plant ingredients
- Development of hormonal, menopause or bone health ranges
- Bioavailability or efficacy studies of isoflavones
- Quality control in the context of certifications or export
Why use YesWeLab?
At YesWeLab, we offer you a tailor-made analytical service, working in partnership with a network of laboratories rigorously selected for their expertise in phytochemistry. Most of our partners are ISO 17025 certified, and some are COFRAC accredited, guaranteeing results that meet international standards.
By choosing YesWeLab, you benefit from:
- Access to cutting-edge techniques (HPLC, LC-MS/MS)
- Personalized technical support
- Optimized deadlines for your R&D projects or product launches
- Digital monitoring from the YesWeLab platform, from request to receipt of results
Recommended internal links
To complete your analytical strategy around isoflavones and related compounds, also consult:
- Daidzin dosage : the glycosylated precursor form of daidzein
- Genistein dosage : another major isoflavone in soy
- Total isoflavone assay : overall quantification of phytoestrogens in extracts
Contact our team
Since 2020, YesWeLab has been supporting nutraceutical and plant health players in managing their laboratory analyses, ensuring scientific rigor, operational simplicity, and transparency. Contact us to obtain a personalized quote or discuss your analytical projects.
