Cyanidin assay

Cyanidin is an anthocyanin pigment naturally present in many red fruits and colored vegetables such as cherries, blackberries, blackcurrants, and red cabbage. It belongs to the flavonoid family and is distinguished by its powerful antioxidant properties and its role in the authentication of plant extracts.

Cyanidin analysis is essential in the nutraceutical, food, and phytotherapeutic sectors to ensure the quality, efficacy, and stability of formulations containing plant-based ingredients. It is also a highly sought-after marker of naturalness in products based on red berries or natural pigments.

Why measure cyanidin in the laboratory?

The measurement of cyanidin helps to meet several industrial and regulatory objectives:

• Quality control of plant extracts standardized in anthocyanins
• Measurement of indirect antioxidant activity in formulations
• Botanical authentication of certain raw materials (e.g., cherry or elderberry juice)
• Verification of the concentration in powders, capsules or fortified drinks
• Comparison between batches or suppliers in a quality context

It can also serve as an indicator of pigment degradation during storage or the manufacturing process.

Analytical methods used

The analysis is performed by high-performance liquid chromatography (HPLC-DAD) or by LC-MS/MS for greater sensitivity and specificity:

• HPLC-DAD: detection by absorbance at 520 nm, specific quantification after chromatographic separation.
• LC-MS/MS: a more precise method allowing the distinction of free cyanidin from its glycosylated or conjugated forms.

The method is validated for complex matrices, including polyphenol-rich extracts, juices, or dry products.

Matrices involved

Cyanidin analysis is applicable to a wide variety of plant-based products, including:

• Extracts of red fruits (cherry, blackberry, raspberry, blackcurrant, blueberry)
• Juices, purees, concentrated syrups
• Berry powders (freeze-dried, dried)
• Capsules, tablets and food supplements
• Enriched liquid formulations
• Raw materials of the natural dye type

Industrial applications

Sectors commonly using this analysis include:

• Nutraceutical industry: valorization of antioxidant extracts
• Food industry: control of natural colorings or labeling of ingredients of plant origin
• Herbal medicine: identification of the raw material
• Cosmetics: products based on natural pigments or for anti-aging activity

Why use YesWeLab?

At YesWeLab, we connect you with specialized phytochemistry analysis laboratories, rigorously selected for their certifications (ISO 17025, COFRAC, etc.) and their expertise in chromatography.

We help you choose the method best suited to your matrix, to prepare your quality or regulatory files, and to interpret the results according to your R&D, marketing or legal needs.

Our digital platform simplifies the ordering, tracking and management of samples in a smooth and fast manner.

Do you need to measure the cyanidin content in your plant products?

Contact our scientific team today for a personalized quote or to discuss your analytical specifications.

And also:

• Total anthocyanin levels
• Safranal dosage
• Flavonoid dosage
• Coumarin dosage
• Dosage of cyanidin-3-O-glucoside