Collagen (hydroxyproline) dosage

Collagen is an essential structural protein found in the connective tissues of animals. It plays a key role in skin firmness , joint health , and muscle structure . One of the most reliable ways to assess collagen content is by measuring hydroxyproline , an amino acid specific to collagen, which is used as an indirect marker of its concentration.

assay (via hydroxyproline) allows control of the quality, purity and conformity of products containing collagen, whether of animal, marine or vegetable , in the food, nutrition or cosmetics sectors.

Analytical methods: HPLC and spectrophotometry

Two main approaches are used for the measurement of hydroxyproline:

• Spectrophotometry : after acid hydrolysis of collagen, the formation of a colored complex is measured at a specific wavelength (generally 560 nm). This simple and rapid method is suitable for routine monitoring .
• HPLC (high-performance liquid chromatography) : allows for more precise quantification of free or bound hydroxyproline, particularly in complex matrices.

These methods guarantee a reliable and reproducible measurement of collagen content, in accordance with standardized analytical recommendations (AOAC, ISO).

Industrial and regulatory applications

Hydroxyproline measurement is used in various fields:

• Nutraceutical industry and health : quality assessment of hydrolyzed collagen powders, peptides and gelatins.
• Human nutrition : control of collagen content in broths, cured meats, confectionery or meat products.
• Animal nutrition : verification of protein intake in collagen-enriched formulations.
• Cosmetics : analysis of the composition of creams, capsules and anti-aging treatments based on collagen.
• Research and development : bioavailability studies and validation of nutritional or functional claims.

This service can be complemented by amino acid assays or total protein analysis for a complete characterization of the protein profile .

Affected matrices

Our partner laboratories perform collagen assays on various matrices:

• Hydrolyzed collagen powders or peptides
• Food products enriched with collagen (bars, drinks, supplements)
• Cosmetics (creams, serums, masks, capsules)
• Foods of animal or marine origin
• Nutritional formulations or veterinary products

Each protocol is tailored to the matrix and the required level of precision , ensuring results that comply with regulatory and industrial .

YesWeLab expertise

At YesWeLab , we collaborate with a network of more than 200 partner laboratories located in France, Europe and internationally , selected for their technical expertise , their ISO 17025 and COFRAC accreditations , and their mastery of nutritional and physico-chemical analyses .

Our scientific team will guide you in choosing the most suitable method , selecting the optimal laboratory , and providing comprehensive monitoring of your analyses via our digital platform . You can centralize your requests , obtain quick quotes , and track your samples online .

Since 2020, many manufacturers, formulators and R&D laboratories have trusted us to guarantee the traceability and conformity of their collagen-based products.

Order a collagen (hydroxyproline) analysis

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