Coenzyme dosage (CoQ9/CoQ10)

CoQ9/CoQ10 assay is a physicochemical analysis used to identify and quantify these ubiquinones in various matrices, such as dietary supplements, nutraceutical formulations, cosmetic products, and plant-based raw materials. Coenzymes Q9 and Q10 are lipophilic compounds involved in the mitochondrial respiratory chain, recognized for their antioxidant properties and their key role in cellular energy metabolism.

Controlling these molecules is crucial to guaranteeing the quality of ingredients, validating claimed concentrations, and ensuring product compliance with regulatory requirements and stated claims.

In what cases should a CoQ9/CoQ10 assay be performed?

CoQ9/CoQ10 assays address numerous industrial and regulatory needs. They allow for quality control of raw materials and active ingredients, validation of the actual content of a food supplement or nutraceutical formulation against labeling, and evaluation of the compound's stability in complex matrices such as oils, capsules, or powders.

This analysis also allows for the differentiation between the oxidized and reduced forms of the compound (ubiquinone/ubiquinol), a crucial distinction because these two forms exhibit different bioavailabilities and functional properties. It is relevant both during the development of new formulations and in continuous quality control processes or the validation of health claims.

What analytical techniques are used for the measurement of CoQ9/CoQ10?

The measurement of coenzyme CoQ9/CoQ10 relies on liquid chromatography techniques, recognized for their sensitivity and specificity towards lipophilic compounds.

High-performance liquid chromatography (HPLC or UPLC), coupled with UV detection, is a reference method for routine quantification and standardization of extracts. Liquid chromatography coupled with mass spectrometry (LC-MS/MS), on the other hand, offers fine structural identification and highly sensitive quantification, particularly suited to complex matrices and the precise differentiation between ubiquinone and ubiquinol. The choice of technique depends on the nature of the sample, the target compounds, and the required quantification limits.

Which sectors are affected?

CoQ9/CoQ10 analysis is primarily relevant to the nutraceutical and human food sectors, where controlling the levels of active ingredients is crucial for product quality and compliance. The animal nutrition, cosmetics, and herbal medicine industries also use it to validate their formulations, standardize their plant extracts, and meet the expectations of both their customers and regulatory authorities.

Additional analyses

For a comprehensive characterization of your products, CoQ9/CoQ10 analysis can be combined with other analyses available in our catalog: antioxidant analysis (vitamins C and E, polyphenols, BHA/BHT) to assess overall protective potential, total antioxidant status (TAS) analysis for a comprehensive view of antioxidant properties,vitamin analysis for nutritional monitoring, and adjuvant analysis for characterizing additives and preservatives. These analyses provide a complete picture of the quality, stability, and compliance of your formulations.

Perform your CoQ9/CoQ10 coenzyme assay with YesWeLab

At YesWeLab, we collaborate with a rigorously selected network of laboratories, most of which are certified and/or accredited (ISO 17025, COFRAC, etc.). These laboratories are chosen based on your specific needs, the matrices to be analyzed, and the required analytical techniques or methods.

If you have specific requirements, please do not hesitate to specify them in your request: our scientific team will do everything possible to respond accurately and promptly. You will also benefit from access to a catalog of over 10,000 analyses as well as personalized scientific support throughout your project.

Since 2020, many manufacturers, distributors and design offices have trusted us to manage their analyses, entrusting us with their samples via our digital platform.

To learn more or to submit a specific need, contact our team now.