Chlorogenic acid dosage

Chlorogenic acid is a polyphenol naturally present in many plants, particularly green coffee, apples, artichokes, sunflowers, and certain medicinal herbs. It is an ester of caffeic acid and quinic acid, belonging to the hydroxycinnamic acid family. Its interest in nutraceuticals and functional nutrition is based on its antioxidant and anti-inflammatory properties and its beneficial effects on the regulation of carbohydrate and lipid metabolism.

Chlorogenic acid measurement is therefore essential to ensure the quality, efficacy, and compliance of plant extracts or health-related formulations. It also serves as a marker of authenticity and standardization for many natural ingredients used in food supplements or fortified food products.

A reliable method based on liquid chromatography (HPLC-DAD, LC-MS/MS)

Chlorogenic acid is usually measured using high-performance liquid chromatography (HPLC) with diode array detection (DAD) or coupled to mass spectrometry (LC-MS/MS). These techniques allow accurate detection, even at low concentrations, and ensure good selectivity over other polyphenols present in the matrix.

Depending on the nature of the samples and the level of quantification sought, the method can be adapted:

• HPLC-DAD for concentrated extracts or routine quality controls.
• LC-MS/MS for complex matrices or trace level analyses.

The methods used are generally validated according to laboratory quality standards (ISO 17025, GMP/GLP if required) and can be adapted to regulatory specifications (e.g. nutritional claims, EFSA, Codex Alimentarius).

Commonly analyzed matrices

Chlorogenic acid measurement is relevant in a wide range of natural and processed products, including:

• Green coffee extracts, particularly in slimming or antioxidant supplements
• Herbal food supplements (artichoke, mate, apple, etc.)
• Herbal teas, infusions and plant powders
• Antioxidant formulations in health nutrition
• Functional food products enriched with polyphenols

Why measure chlorogenic acid?

The interest of the dosage is based on several strategic axes:

• Quality control of standardized extracts: verification of the expected concentration of chlorogenic acid in the ingredient or raw material.
• Evaluation of functional claims: Since chlorogenic acid is known for its effect on blood sugar modulation and its antioxidant properties, its presence must be verified to justify certain claims.
• Regulatory compliance: within the framework of legislation on food supplements (e.g.: supervision of active ingredient contents).
• Authentication of plant extracts: detection of the presence or absence of chlorogenic acid as a tracer of a phytocompound profile.

A service tailored to your needs, from R&D to production

At YesWeLab, we provide you with a network of partner laboratories specialized in the analysis of plant active ingredients, equipped with the latest chromatography technologies (HPLC, UPLC, LC-MS/MS). Each request is handled individually according to:

• The matrix to be analyzed (dry extract, liquid, finished product, etc.)
• The expected quantification threshold
• The level of validation required (R&D, production, regulation, etc.)

Our partner laboratories are mostly ISO 17025 certified, and some have specific accreditations depending on your sector (COFRAC, GMP, GLP, etc.).

Have your extracts analyzed with YesWeLab

Since 2020, YesWeLab has been supporting manufacturers, distributors, formulators, and laboratories in managing their analyses thanks to a simple and efficient digital platform. Save time and access reliable, comparable assay services performed as quickly as possible.

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Need a quote or advice on chlorogenic acid analysis? Contact our scientific team now.