Chelidonine (isoquinoline alkaloid) dosage

Chelidonine is an isoquinoline alkaloid found primarily in greater celandine (Chelidonium majus). This molecule is known for its spasmolytic, analgesic, antiproliferative, and cytotoxic properties, making it a compound of major interest to the phytotechnology, nutraceutical, and natural cosmetics industries. Chelidonine is also among the alkaloids that require careful monitoring due to their potential toxicity, particularly in concentrated plant extracts or functional ingredients derived from medicinal plants.

Chelidonin analysis ensures the quality, purity, and safety of plant-based raw materials, and also verifies regulatory compliance for formulations intended for non-therapeutic applications. It is a crucial element in the quality control of botanical extracts, where alkaloid content can vary depending on origin, botanical species, extraction conditions, and purification processes.

Why analyze chelidonine?

Chelidonin analysis is essential for several reasons:
• standardization of celandine extracts or extracts of other plants containing isoquinoline alkaloids
• ensuring the safety of formulations for external use (cosmetics, wellness, natural products)
• evaluation of the phytochemical profile of functional ingredients
• detection of potentially toxic alkaloids in highly concentrated extracts
• complete characterization of ingredients intended for nutraceuticals or R&D
• compliance with regulations applicable to non-medical products containing plant extracts

Thanks to this analysis, manufacturers can guarantee a consistent level of quality while controlling toxicological risks.

Analyzed matrices

YesWeLab supports the assay of celandine in a wide range of matrices:
• raw or purified plant extracts
• celandine powders
• glycerinated macerates and tinctures
• hydroalcoholic or hydroglycolic extracts
• functional ingredients for nutraceuticals
• raw materials for natural cosmetics
• complex formulations containing plant mixtures

Each matrix may require specific preparation (extraction, purification, dilution), carried out according to validated protocols.

Analytical techniques used

Chelidonin is quantified by advanced analytical chemistry methods, including:
• HPLC-DAD (high-performance liquid chromatography with UV detection)
• LC-MS or LC-MS/MS for optimal sensitivity, including in the presence of complex matrices
• selective extraction or SPE purification depending on the nature of the sample

Partner laboratories use standardized methods or validated internal methods in accordance with ISO 17025, ensuring reliable and reproducible results.

Interest in the nutraceutical, plant and cosmetic industries

Chelidonin analysis is particularly relevant for:
• manufacturers of botanical extracts
• producers of herbal food supplements
• formulators of natural and dermo-cosmetic products
• phytochemistry R&D laboratories
• distributors of functional ingredients
• regulatory consulting firms

Controlling alkaloid content is a major challenge to avoid toxicological risks, optimize active concentrations and ensure complete traceability of ingredients.

Additional analyses available

YesWeLab also offers related analyses for a more comprehensive characterization of plant extracts:
• Total polyphenol analysis
• Flavonoid analysis
• Coumarin analysis
• Heavy metals (ICP-MS)
• Pesticide analysis

The YesWeLab service

YesWeLab relies on a network of specialized laboratories, selected for their expertise and often accredited to ISO 17025 or COFRAC standards. Each request is directed to the most suitable laboratory, based on the matrix, the desired limit of quantification, regulatory requirements, and the necessary analytical techniques.
Since 2020, our digital platform has enabled manufacturers and engineering firms to easily manage their analyses, from request to results. Our scientific team supports you in defining the right methods, interpreting results, or developing a comprehensive analytical plan.