Caryophyllene determination

Caryophyllene is an aromatic bicyclic sesquiterpene naturally present in many essential oils, including clove, black pepper, hops, cinnamon, and rosemary. It contributes to the olfactory and taste profiles of plant extracts and also plays an important biological role thanks to its anti-inflammatory, analgesic, and antioxidant properties. Its interest is growing in the nutraceutical, cosmetic, and food industries, both for its aromatic qualities and its functional effects.

Caryophyllene analysis allows us to characterize its concentration in an essential oil or formulation, to verify the authenticity of an extract, to ensure regulatory compliance and to standardize the quality of finished products.

Principle of caryophyllene dosage

The dosage of caryophyllene is based on high-performance chromatographic techniques:

• GC-FID (Gas Chromatography with Flame Ionization Detection) : Used for the precise quantification of sesquiterpenes, suitable for essential oils and volatile extracts.
• GC-MS (gas chromatography-mass spectrometry) : allows reliable identification of caryophyllene, even in the presence of complex matrices or other structurally close sesquiterpenes.

These methods guarantee reproducible quantification, high specificity and traceability in accordance with analytical and regulatory standards.

Industrial applications

Caryophyllene dosage is essential for many industrial sectors:

• Nutraceuticals : evaluation of caryophyllene content in herbal food supplements (clove, turmeric, cannabis, black pepper), used for their anti-inflammatory and antioxidant effects.
• Cosmetics and personal care : quality control of essential oils and extracts incorporated into creams, balms or lotions, for their soothing and protective properties.
• Aromatherapy : verification of the authenticity and composition of essential oils used in therapeutic practices.
• Food industry : dosage in natural flavors and food formulations to standardize taste and ensure compliance with safety standards.
• Research and innovation : characterization of caryophyllene in pharmacological, biochemical and valorization studies of new plant raw materials.

Affected matrices

The matrices typically analyzed in the context of caryophyllene determination are:

• Pure essential oils or complex blends
• Plant extracts (cloves, black pepper, hops, etc.)
• Food supplements and nutraceutical formulations
• Cosmetic products (oils, lotions, creams, perfumes)
• Food flavors and fortified beverages
• Experimental pharmaceutical and galenic preparations

Quality and compliance issues

The determination of caryophyllene meets several strategic objectives:

• Authenticity and traceability : detection of possible adulterations or falsifications in essential oils.
• Standardization : guarantee of a constant caryophyllene content in formulations intended for the market.
• Safety and regulatory compliance : compliance with current thresholds and directives for food, cosmetic and nutraceutical products.
• Commercial promotion : highlighting functional properties and specific chemical profiles in a marketing logic.

YesWeLab support

YesWeLab offers you its network of partner laboratories specializing in the chromatographic analysis of essential oils and plant extracts. Most of our partners are certified and accredited (ISO 17025, COFRAC), guaranteeing the reliability of their results and their compliance with international standards.

With YesWeLab, you benefit from:

• Fast and easy online submission of your samples
• Real-time monitoring of analyses via our digital platform
• Reliable, reproducible results that meet regulatory expectations
• Support from our scientific team in choosing the analytical methods best suited to your matrices

Related analyses

• Monoterpene dosage
• Camphor Dosage
• Dosage of 1,8-cineole (eucalyptol)
• Total terpene assay
• Profiling of volatile compounds
• Analysis of flavonoids and polyphenols