Bioavailability assay

Bioavailability testing determines the actual proportion of an active compound or nutrient that is readily available for absorption after ingestion. This analysis is essential for evaluating the performance and efficacy of an active ingredient, particularly in the cosmetic, nutraceutical, and nutritional sectors. It allows for the comparison of different formulations, the validation of scientific claims, and the optimization of active ingredient release in products intended for consumption or topical application. Understanding the bioavailability of an active ingredient also helps guide formulation choices, adjust dosages, and ensure the scientific consistency of innovative products.

Analytical methods

Bioavailability assessment most often relies on in vitro digestion methods that replicate the physiological conditions of the digestive tract. These models include gastric and intestinal phases, allowing observation of the release, solubilization, and availability of the active ingredients. The released compounds are then quantified by liquid chromatography (HPLC or UPLC) or mass spectrometry (LC-MS), offering high sensitivity and the ability to precisely distinguish the active ingredients of interest within complex matrices. This approach makes it possible to monitor the degradation, transformation, or gradual release of the active molecules, while simultaneously evaluating their actual absorption potential.

Analyzed matrices

Bioavailability testing can be applied to various product categories, including dietary supplements, nutritional formulations, standardized plant extracts, products enriched with vitamins, polyphenols, or minerals, and certain cosmetic formulations when assessing the skin bioavailability of an active ingredient. The results provide valuable insights into the actual behavior of ingredients, their efficacy, and their stability in the final matrix.

Interest for professionals

For companies developing dietary supplements, plant extracts, or functional cosmetic products, bioavailability testing is a key performance indicator. It allows them to optimize formulations, choose the right absorption vectors, support marketing claims, and demonstrate the efficacy of an active ingredient. It also meets the needs of R&D teams wishing to compare several prototypes or measure the impact of new encapsulation, emulsion, or solubilization technologies. For distributors and manufacturers, this analysis strengthens the scientific credibility of their products and ensures optimal quality control.

YesWeLab Expertise

YesWeLab relies on a network of laboratories specializing in physicochemical analysis and in vitro digestion, selected for their technical expertise and often accredited according to ISO 17025 or COFRAC standards. Depending on your needs, our team guides you toward the most relevant methods, oversees sample management, and ensures comprehensive follow-up until results are delivered. Since 2020, we have been supporting manufacturers, formulators, R&D laboratories, and distributors in the quality control and scientific validation of their products.

Other relevant analyses from the YesWeLab catalogue

The following services can complement bioavailability testing:

• In vitro digestion of other compounds
• Polyphenol and vitamin dosage
• Stability studies of formulations