Dosage of baicalein (flavone)

Baicalein analysis quantifies this bioactive flavone, primarily derived from Scutellaria baicalensis, a plant widely used in traditional herbal medicine and nutraceuticals. Baicalein is recognized for its antioxidant, anti-inflammatory, neuroprotective, and antimicrobial properties. Its presence and concentration in plant extracts or finished formulations must be controlled to ensure product quality, standardization, and efficacy.

Industrial challenges of baicalein dosage

In the nutraceutical and functional plant ingredients sectors, controlling baicalein levels is essential to ensure batch conformity, product homogeneity, and the validity of associated claims. It also serves as a quality indicator for raw materials, particularly when used as a chemical marker for the standardization of Scutellaria extracts.

The analysis allows us to:
• verify the purity and authenticity of plant extracts
• ensure the consistency of a formulation incorporating flavones and flavonoids
• monitor potential degradation during extraction or drying processes
• evaluate the stability of baicalein in complex matrices
• secure the supply chain of natural ingredients

Analytical methods: HPLC and LC-MS

Baicalein is primarily quantified by high-performance liquid chromatography (HPLC), often coupled with DAD detection for precise spectral identification. For more demanding requirements, particularly in the presence of structurally similar molecules or complex matrices, LC-MS or LC-MS/MS offers superior sensitivity and selectivity.

These methods allow for:
• precise quantification of baicalein
• differentiation between baicalin and baicalein
• detection of isomers or related compounds
• validation of conformity according to current analytical standards

Preliminary extraction or purification steps may be necessary depending on the matrix being analyzed (plant powder, dry extract, hydroalcoholic solution, food supplement, etc.).

Matrices analyzed and applications

Baicalein dosage concerns a wide range of products related to medicinal plants and active ingredients: extracts of Scutellaria baicalensis, plant powders, standardized concentrates, nutraceutical formulations, liquid or gelled supplements, raw materials intended for the manufacture of functional products.

Its applications include:
• standardization of plant extracts
• quality control of imported raw materials
• validation of antioxidant or soothing claims
• research and development of new flavonoid complexes
• stability studies during storage or industrial processes

YesWeLab Support

YesWeLab relies on an expert network of laboratories specializing in chromatographic, physicochemical, and nutraceutical analyses. The majority of our partners are certified or accredited according to ISO 17025 and COFRAC standards, guaranteeing reliable, reproducible measurements that comply with industry standards. Our scientific team helps you define the most suitable method, select the test matrix, anticipate potential interferences, and interpret the results.

Thanks to our digital platform, you can easily manage your analysis orders, track your samples, and access your reports. Since 2020, numerous players in the nutraceutical, phytotherapy, and plant research sectors have trusted us to ensure the quality of their bioactive extracts.

Other analyses from the YesWeLab catalogue

• Baicalin assay
• Total flavonoid assay
• Polyphenol analysis