Bacillus cereus dosage

Bacillus cereus testing is an essential step in the microbiological control of food products, plant-based ingredients, and nutritional supplements. This spore-forming and ubiquitous bacterium is known for its ability to produce toxins that cause food poisoning. Its detection ensures product safety and compliance with regulatory requirements.

The analysis is based on enumeration on specific culture medium, quantitative PCR and DNA sequencing (NGS), which ensure accurate and rapid identification of B. cereus and their potential toxic genes.

Principle and operation of the method

The sample (powder, flour, plant extract, or dietary supplement) is homogenized and then inoculated onto a selective medium that promotes the growth of Bacillus cereus . After incubation, the count is expressed in colony-forming units (CFU/g), allowing for the assessment of the total microbial load.
In addition, PCR can detect the presence of genes encoding emetic and diarrheal toxins, providing a more comprehensive approach to the health risk. Next-generation sequencing (NGS) can also be used to identify strains and trace contamination of environmental or industrial origin.

This combination of cultural and molecular methods ensures reliable detection, including in complex or slightly contaminated matrices.

Analytical performance and related analyses

Bacillus cereus testing is a sensitive and specific method for rapidly identifying high-risk microbial contamination in dry goods and ingredients. It is part of good hygiene practices and HACCP control.

For a comprehensive assessment of microbiological safety, this analysis can be combined with:

• coliform assay,
• the enumeration of Escherichia coli,
• the enumeration of yeasts and molds,
• or even the search for Salmonella spp.

These additional tests make it possible to verify the overall regulatory compliance of finished products and plant-based raw materials, particularly in sectors with high health requirements.

Applications and areas of use

The analysis of Bacillus cereus is applicable to many fields:

• Human food : microbiological monitoring of processed products, flours, protein powders, spices, infant milks or prepared meals.
• Nutraceuticals : quality control of natural extracts and food supplements based on plants or cereals.
• Animal nutrition : verification of the microbiological cleanliness of raw materials and premixes intended for animal feed.
• Plants and plant ingredients : analysis of contamination of powders and botanical extracts in order to prevent any health risks when incorporating them into formulations.

These controls are often supplemented by analyses such as antioxidant dosage or heavy metal testing to ensure overall product safety.

The advantages of the YesWeLab service

YesWeLab relies on a network of partner laboratories specializing in microbiology, accredited to ISO 17025 and COFRAC standards. Each request is directed to the laboratory best suited to the matrix, the method, and the required level of expertise.

Thanks to its digital analysis platform, YesWeLab simplifies ordering, tracking, and receiving results. Since 2020, numerous manufacturers, formulators, and engineering firms have trusted us for their microbiological testing, guaranteeing reliability, responsiveness, and regulatory compliance.

For any specific request relating to the dosage of Bacillus cereus , contact our scientific team to obtain personalized support and a quote tailored to your needs.