Assay of salidroside (phenol) and rosavin derivatives (rosavin)

The measurement of salidroside and rosavin derivatives is an essential analytical tool for the characterization, quality control, and standardization of plant extracts from Rhodiola rosea . These phenolic compounds are among the main active markers of rhodiola, an adaptogenic plant widely used in the nutraceutical and plant-based ingredient sectors.

The precise quantification of salidroside and rosavins ensures the quality of raw materials, the consistency of formulations and the security of analytical data used in technical, quality and regulatory files.

Principle of analysis

Salidroside is a glycosylated phenol, while rosavins encompass several related compounds, including rosavin, rosarin, and rosin. These molecules are naturally present in the roots of Rhodiola rosea and are key indicators of the authenticity and quality of extracts.

The analysis is based on a suitable extraction of the plant matrix, followed by chromatographic separation allowing for reliable identification and quantification of each target compound. Depending on the product specifications, the results can be expressed individually or as an overall rosavin content.

Analytical methods used

The assay of salidroside and rosavin derivatives is carried out by high-performance liquid chromatography (HPLC), usually coupled with UV-visible or diode array detection, or by liquid chromatography coupled with mass spectrometry (LC-MS or LC-MS/MS).

HPLC is particularly well-suited for quantifying rhodiola markers in well-characterized matrices and offers excellent reproducibility of results.
LC-MS is preferred when lower limits of quantification are required, when the matrix is ​​complex, or when formal identification of compounds is necessary, particularly for differentiating between species or botanical origins.

The choice of method is defined in advance by the YesWeLab scientific team in order to guarantee the relevance and reliability of the results.

Measured parameters

This analysis makes it possible to determine the salidroside content, the rosavin content and other rosavin derivatives, the total rosavin content when required, as well as the comparison between batches, suppliers or formulations.

Analytical results provide an objective basis for quality control, raw material qualification and finished product validation.

Matrices involved

The assay of salidroside and rosavins applies to a wide range of matrices, including dry rhodiola extracts, vegetable powders, capsules, tablets and other standardized nutraceutical products.

Each matrix undergoes specific preparation to limit interferences and ensure accurate and reproducible quantification of the targeted compounds.

Industrial applications

This analysis is widely used in the nutraceutical sector for quality control of raw materials, standardization of Rhodiola rosea , and supplier qualification. It is also used in the development and reformulation of adaptogenic products to ensure consistent active ingredient levels.

In research and development, the measurement of salidroside and rosavins makes it possible to compare different extraction methods, to evaluate the impact of transformation processes and to optimize the concentration of bioactive compounds.

The analysis is also used in investigative procedures in cases of non-compliance, suspected adulteration, or variation in analytical profile.

Sectors concerned

This service is primarily aimed at nutraceutical players, plant and plant-based ingredient sectors, as well as laboratories, design offices and formulators working on adaptogenic extracts.

Quality and compliance issues

Analytical characterization of salidroside and rosavins is essential for ensuring the accuracy of product specifications, technical dossiers, and compositional data used in commercial and regulatory communications. Reliable analysis guarantees the authenticity, traceability, and quality of rhodiola extracts.

YesWeLab Expertise

YesWeLab relies on a rigorously selected network of partner laboratories, most of which are certified and/or accredited according to recognized standards such as ISO 17025 and COFRAC. Each project is analyzed by our scientific team to guide samples toward the analytical method best suited to the client's objectives and the specific constraints of the matrices.

Thanks to its digital platform, YesWeLab enables centralized analysis management, optimized sample tracking, and fast, secure access to analytical results. Since 2020, numerous manufacturers, distributors, and research firms have trusted YesWeLab for their physicochemical and nutritional analyses.

Other analyses from the YesWeLab catalogue

The dosage of salidroside and rosavins can be complemented by other analyses from the YesWeLab catalogue in order to obtain a global characterization of plant extracts and adaptogenic products.

The measurement of total polyphenols allows the active ingredients of rhodiola to be placed in a global antioxidant context.

The measurement of flavonoids and tannins complements the evaluation of phenolic compounds present in plant matrices.

The dosage of rhein and hydroxyanthracenes , the dosage of salicylaldehyde or even the dosage of xanthones can be relevant in complex formulations combining several botanical extracts.

HPLC and LC-MS or LC-MS/MS analyses are also offered for the separation and quantification of many bioactive compounds of plant origin.

Finally, nutritional analyses allow the dosage of salidroside and rosavins to be integrated into a comprehensive approach to the quality and compliance of nutraceutical products.

For any quote request or to discuss your specific needs in salidroside and rosavin derivatives dosage, contact the YesWeLab team now.