EDTA (ethylenediaminetetraacetic acid) assay

What is EDTA?

EDTA (ethylenediaminetetraacetic acid) is a chelating agent widely used in cosmetic, food, pharmaceutical, and industrial formulations. Its main function is to complex metal ions, thereby preventing their catalytic, oxidative, or precipitating action. This helps to prolong product stability, enhance the effectiveness of preservatives, and optimize industrial processes sensitive to trace metals.

It is found in shampoos, creams, disinfectants, food supplements, cleaning products, and water treatments. In the form of free acid or salts (Na₂EDTA, CaNa₂EDTA…), its presence is strictly regulated according to the sectors of application.

Why measure EDTA?

The dosage of EDTA is essential for:

• Verify compliance with formulation specifications
• Respect regulatory limits according to usage (e.g., cosmetics, food)
• Control the quality of raw materials and finished products
• Validate the product's effectiveness from a technological or preservative standpoint
• Documenting authorization or registration files (SDS, CPNP, REACH)

Some regulations impose maximum permitted levels, particularly in cosmetics (EC Regulation No. 1223/2009) or in certain food uses, where EDTA is listed as an additive (E385) under certain conditions.

Which matrices are involved?

EDTA dosage is applicable to a wide variety of industrial and commercial products:

• Cosmetic formulations: creams, gels, hair care products, cleansers
• Agri-food products: sauces, canned goods, drinks, supplements
• Pharmaceutical products or medical devices
• Disinfectants, cleaning solutions, detergents
• Process or treatment water (particularly in the textile or chemical industries)

Analytical methods for EDTA determination

Several reliable analytical methods allow for the quantification of EDTA depending on the nature of the matrix:

• Complexometric titration (with Mg²⁺ or Ca²⁺ ions): a classical method suitable for simple solutions
• HPLC (High-Performance Liquid Chromatography): enables efficient separation even in complex formulations
• UV-Visible Spectrophotometry: after color derivatization or complexation, suitable for products at low concentrations
• Methods validated according to ISO, USP, or internal standards according to ISO 17025

The choice of method depends on the type of sample, the required precision, regulatory thresholds and the context of use.

YesWeLab: your partner for EDTA analysis

At YesWeLab , we connect you with expert laboratories specializing in precise chemical analysis, capable of quantifying EDTA in simple or complex matrices. Our partners are ISO 17025 certified and often COFRAC accredited, ensuring a high level of expertise and complete traceability of results.

We support you in:

• Define the analytical method best suited to your needs
• Ensure the regulatory compliance of your finished products or raw materials
• Obtain actionable analytical reports for auditing or regulatory purposes
• Benefit from personalized support provided by our scientific team

Our secure digital platform allows you to centralize all your analyses, track your projects in real time, and view your results at any time.

Need a reliable and standardized EDTA dosage?

YesWeLab supports you in the quality, regulatory, and technical control of your EDTA-containing formulations.
Contact our team today for a quote or to discuss your specific chemical analysis needs.