Assay of amylisonitrite and isoamyl nitrite

Amylisonitrite (or isoamylnitrite) and isoamyl nitrite are two volatile organic compounds derived from nitrites, historically used for their vasodilatory properties in some traditional pharmacopoeias. They are also found in technical products, solvents, and industrial herbal formulations, notably for their muscle-relaxing effects or as flavoring agents. However, their potential toxicity and misuse in recreational contexts have led to stricter regulatory oversight.

The measurement of amylisonitrite and isoamyl nitrite is now essential to verify the absence or conformity of these substances in nutraceutical products, plant extracts, or technical liquids.

Why analyze amylisonitrite and isoamyl nitrite?

These substances belong to the nitrite ester family, known to cause rapid vasodilation when inhaled or ingested. Although some medical uses have been documented in the past (notably for the treatment of angina pectoris or as an antidote to cyanide poisoning), their use is now strictly regulated, or even prohibited in many countries.

Their euphoric effect, combined with cardiovascular, neurological, and metabolic risks, makes them targets of regulatory monitoring. Therefore, manufacturers must demonstrate their absence or control in formulations where their presence might be suspected: imported products, traditional plant extracts, botanical solvents, etc.

Available analytical methods

YesWeLab offers several reliable and sensitive assay approaches, depending on the matrix:

• GC-FID (gas chromatography with flame ionization detection): suitable for detecting low concentrations in solvents or volatile bases.
• GC-MS (gas chromatography coupled with mass spectrometry): reference method for the precise identification of trace compounds in complex matrices.
• HPLC (high-performance liquid chromatography): used for heat-sensitive or soluble samples in aqueous or glycerol matrices.

Our methods allow us to precisely quantify these compounds, often below ppm, in technical or nutraceutical matrices.

Matrices involved

The dosage is particularly suitable for the following matrices:

• Traditional or ethnobotanical plant extracts
• Botanical chemicals and solvents
• Liquid preparations or mother tinctures
• Technical or cosmetic formulations containing nitrite derivatives
• Products that can be used for other purposes (aromatherapy, recreational uses)

Further analyses recommended

To ensure complete quality control and enhanced regulatory compliance, it is advisable to combine this assay with other analyses targeting hazardous compounds or residual solvents:

• Residual solvent analysis (GC) – to detect volatile impurities in technical products or plant extracts.
• Nitrite and nitrate dosage – as a supplement in food or phytotherapeutic matrices.

Safety, compliance and regulations

European regulations prohibit or severely restrict the presence of nitrite esters in food, nutraceutical products, and preparations intended for the general public. Some authorities, such as the EFSA and the ANSM, consider these compounds potentially dangerous in cases of chronic exposure or misuse. Analytical traceability therefore becomes an essential tool for meeting the requirements of distributors, exporters, and regulatory authorities.

The dosage proposed by YesWeLab is part of this logic of risk prevention, consumer protection, and regulatory compliance.

YesWeLab: your partner for specific dosages

YesWeLab provides you with access to its network of accredited partner laboratories (ISO 17025, COFRAC, etc.), experts in physicochemical analysis and the testing of regulated substances. Our scientific teams advise you on the techniques best suited to your matrix and your objectives.

Add this test to your quote request now or contact our team for personalized support.