Annonacin Dosage

Annonacin analysis allows for the quantification of this alkaloid found in several plants of the Annonaceae family, notably graviola (Annona muricata) and soursop. Annonacin belongs to the acetogenins, a group of natural compounds with marked biological activity, but also known for their neurotoxic potential at high doses. Its analytical control is therefore essential in plant extracts, dietary supplements, and nutraceutical formulations containing these plants.

Industrial challenges and safety of use

Annonacin is at the heart of numerous safety concerns related to graviola and Annonaceae products. Some studies have highlighted a link between high chronic exposure to these acetogenins and neurotoxic risks. Manufacturers must therefore ensure that their products do not contain potentially dangerous levels, while guaranteeing quality and regulatory compliance.

The analysis allows us to:
• verify the absence or low concentration of annonacin in supplements
• ensure the safe marketing of graviola-based products
• control batches of imported plant-based raw materials
• anticipate regulatory requirements for acetogenins
• document quality procedures and technical files

This analysis is particularly important for players in nutraceuticals, herbalism, plant extracts and functional ingredients.

Analytical methods: LC-MS and HPLC

Annonacin assay is based primarily on liquid chromatography coupled with mass spectrometry (LC-MS or LC-MS/MS), the most sensitive and specific method for detecting this molecule in complex matrices such as plant extracts, powders or formulated supplements.

HPLC can also be used for certain types of samples, although it offers lower sensitivity compared to LC-MS/MS. The analysis often requires a specific preparation step to extract acetogenins and eliminate natural interferences present in plants.

These methods allow:
• precise quantification even at very low concentrations
• detection of other acetogenins if necessary
• reliable interpretation in a public health safety context

Matrices analyzed and applications

The dosage is applicable to many matrices related to Annonaceae: dry extracts, vegetable powders, dried leaves, infusions, food supplements, juices, liquid or gelled formulations.

Key applications include:
• quality control of graviola raw materials
• consumer safety risk assessment
• validation of marketed formulations
• comparative studies between suppliers
• compliance with internal standards or international recommendations

YesWeLab Support

YesWeLab relies on a network of laboratories specializing in LC-MS/MS, plant analysis, and nutraceuticals. The majority of our partners are certified or accredited according to ISO 17025 and COFRAC standards, guaranteeing reliable, reproducible, and scientifically robust results. Our scientific team supports you in:
• choosing the appropriate method
• preparing samples
• interpreting results
• implementing quality control plans

Since 2020, many distributors, nutraceutical formulators and importers of natural ingredients have trusted us to secure their Annonaceae-based products and ensure the conformity of their batches.