Analysis of oxygenated metabolites (oxylpines)

The analysis of oxygenated metabolites , also known as oxylipins, allows for the characterization of a family of bioactive compounds derived from the enzymatic or non-enzymatic oxidation of polyunsaturated fatty acids. These molecules play a key role in the regulation of inflammation, oxidative stress, cell signaling, and numerous physiological processes. Their measurement is essential for understanding the biological activity of a plant extract, oil, nutraceutical ingredient, or food matrix. This analysis is also a valuable tool for evaluating the anti-inflammatory and functional properties of natural products.

Importance of analysis

Oxylipins are central to multiple metabolic pathways involving lipoxygenases, cyclooxygenases, and cytochrome P450 enzymes. Oxylipin profiles allow for the evaluation of the quality, activity, and efficacy of ingredients rich in polyunsaturated fatty acids. This analysis is used to: compare the anti-inflammatory activity of different extracts, improve the development of nutraceutical formulations, monitor the oxidative stability of vegetable or marine oils, understand the biological mechanisms related to the inflammatory response, and select raw materials with high functional value. For manufacturers, it provides a key indicator for maximizing the value of natural ingredients and demonstrating their functional benefits.

Analytical methods

The reference technique for oxylipin analysis is liquid chromatography coupled with mass spectrometry (LC-MS or LC-MS/MS). It allows:

• the precise identification of oxygenated metabolites
• quantification at very low concentrations
• the distinction between different fatty acid derivatives: HODE, HETE, resolvins, prostaglandins, leukotrienes, oxo-DHA, etc.
• simultaneous analysis of multiple metabolic pathways

LC-MS/MS methods must be adapted to the matrix to ensure efficient extraction and prevent secondary oxidation. This technique offers a high level of sensitivity, essential for trace molecules.

Analyzed matrices

Oxylipin analysis is applicable to a wide range of plant-based or nutraceutical matrices: vegetable oils (flaxseed, hemp, camelina, perilla), marine oils (EPA/DHA), plant extracts, plant powders, functional foods, lipid ingredients, fortified food matrices, fermented products, and certain biological matrices of plant origin. Each matrix requires specific preparation to preserve oxidation-sensitive metabolites.

Interest for professionals

Manufacturers of functional ingredients, nutraceutical formulators, food processors, and research laboratories use this analysis to optimize their formulations, assess product stability, document functional benefits, validate raw material quality, and characterize biological activity. Oxylipin analysis also allows for objective comparison of different batches, supports clinical and preclinical studies, and substantiates claims related to anti-inflammatory or antioxidant activity.

YesWeLab Expertise

YesWeLab collaborates with laboratories specializing in lipidomics and bioactive metabolite analysis. The majority of our partners are ISO 17025 or COFRAC certified or accredited, guaranteeing the quality of the results. We select the most suitable method for your matrix and objective (comprehensive profiling, targeted quantification, comparative analysis). Our digital platform provides seamless tracking, simplified sample management, and responsive scientific support. Since 2020, numerous manufacturers and research firms have entrusted us with the analysis of their plant extracts, oils, and functional ingredients.

Other relevant analyses from the YesWeLab catalogue

• Fatty acid profile (GC-FID, GC-MS)
• Analysis of polyphenols by LC-MS
• Antioxidant dosage
• Oxidative stability studies of oils