Amylase dosage

What is amylase?

Amylases are hydrolase enzymes responsible for the breakdown of starch into simple sugars (maltose, glucose). We mainly distinguish:

• Alpha-amylases: random cleavage of alpha-1,4 bonds
• Beta-amylases: release maltose at the end of the chain
• Gamma-amylases or glucoamylases: also hydrolyze alpha-1,6 bonds

Their activity is essential in many industrial processes, particularly in nutrition, agri-food, biotechnology, enzyme formulation, and plant production.

Why measure amylase activity?

Amylase dosage allows:

• Evaluate the enzymatic quality of a plant extract or active ingredient
• Validate the functional performance of enzymatic formulations (supplements, premixes, additives)
• Control manufacturing consistency in industrial processes (breadmaking, brewing, starch production)
• Optimize the storage or use conditions of an enzymatic solution
• Study the biological activity of certain plant extracts rich in natural enzymes

This is a critical indicator for applications in human and animal nutrition, but also in the production of functional ingredients or fermented beverages.

Analysis method

The enzymatic activity of amylases is measured by a spectrophotometric enzymatic method, generally based on:

• Colorimetric detection of starch hydrolysis products
• The measurement of the increase in absorbance linked to the formation of reducing sugars or to the use of chromogenic substrates

This method is recognized for its simplicity, reproducibility and precision, and can be adapted to different matrices after extraction.

Affected matrices

Amylase activity analyses apply to various matrices:

• Raw or purified plant extracts
• Enzymatic food supplements (capsules, tablets, powders)
• Technical ingredients or nutritional premixes
• Agri-food or starch raw materials
• Bakery products or industrial preparations
• Stabilized or lyophilized enzyme solutions

Results are usually expressed in units of enzyme activity (U/mg, U/g or U/mL).

Industrial applications

Amylase testing is useful for:

• Quality control and stability of enzyme products
• Targeted formulation based on enzymatic needs (digestion, biotransformation, etc.)
• Development of plant-based digestive supplements
• Monitoring of biotechnological or fermentation processes

It is part of a logic of analytical and functional mastery of products derived from biotechnologies and natural extracts.

Why choose YesWeLab?

YesWeLab centralizes your analysis requests by relying on a network of specialized partner laboratories, selected for their expertise in enzymology, biochemistry and quality control.

We guarantee you:

• Methods validated according to industry standards
• Accredited laboratories (ISO 17025, COFRAC, etc.)
• Rapid support adapted to your production or development constraints
• Personalized scientific support, from method selection to interpretation of results
• A simple digital platform to manage your requests, reports and analysis history

Do you want to measure the amylase activity of your extracts or formulations?

Contact YesWeLab to schedule a personalized analysis or request a quote directly from our platform.